50+ MCQ on Chromatography with Answers PDF
MCQ on Chromatography: Chromatography is a separation technique in which the complex mixture are separated into two phases: a stationary phase with a large surface area, and a mobile phase. The goal of the stationary phase is to delay the passage of the sample components.
When components pass through the system at different speeds, they separate at certain times. Each component has a characteristic time to pass through the system, called the retention time. Chromatographic separation is achieved when the retention time of the analyte differs from the rest of the components of the sample.
Chromatography is one of the main analytical methods and allows the separation and quantification of substances that are very similar in structure and chemical properties.
Chromatography MCQ with Answers PDF
Below you will get MCQ on Chromatography. For the ease of students, we at ybstudy.com provide Chromatography MCQ in PDF format for free. It covers all concepts and all topics of MCQ on Chromatography like paper chromatography, column chromatography, TLC, and Ion exchange chromatography.
These MCQ on Chromatography are prepared by our Biology senior and experienced faculty which gives students an in-depth understanding of the concepts rather than the particular question.
1. Which force is responsible for the separation of the components in descending paper chromatography?
1. Gravity
2. Capillary action
3. Electrostatic force
4. Magnetic force
Answer: 2. Capillary action
2. The size of the spot in paper chromatography is
1. Directly proportional to concentration
2. Inversely proportional to concentration
3. Independent of concentration
4. Unrelated to the separation process
Answer: 1. Directly proportional to concentration
3. Which of the following is used as a spraying reagent in paper chromatography?
1. conc. HCl
2. NaCl solution
3. Ninhydrin solution
4. CuSO4 solution
Answer: 3. Ninhydrin solution
4. Which force is responsible for the separation of the components in descending paper chromatography?
1. Gravitational force
2. Magnetic force
3. Centrifugal force
4. Capillary action
Answer: 4. Capillary action
5. Which force is involved in paper chromatography?
1. Magnetic force
2. Centrifugal force
3. Capillary action
4. Gravitational force
Answer: 3. Capillary action
6. Which force is involved in chromatography?
1. Electrostatic force
2. Magnetic force
3. Capillary action
4. Centrifugal force
Answer: 3. Capillary action
7. Which factor is not affected in stationary phases in paper chromatography?
1. Polarity
2. Particle size
3. Temperature
4. pH
Answer: 3. Temperature
8. In chromatography, the stationary phase can be ___________ supported on a solid.
1. Covalently bonded
2. Hydrogen bonded
3. Ionically bonded
4. Physically adsorbed
Answer: 4. Physically adsorbed
9. Liquid chromatography can be performed in which of the following ways?
1. Gas-liquid chromatography
2. Size exclusion chromatography
3. High-performance liquid chromatography (HPLC)
4. Affinity chromatography
Answer: 3. High-performance liquid chromatography (HPLC)
10. Which type of filter paper is mostly used in paper chromatography?
1. Whatman No. 1
2. Whatman No. 4
3. Whatman No. 6
4. Whatman No. 8
Answer: 1. Whatman No. 1
11. In which of the following types of paper chromatography does the mobile phase move horizontally over a circular sheet of paper?
1. Ascending chromatography
2. Descending chromatography
3. Circular chromatography
4. Radial chromatography
Answer: 4. Radial chromatography
12. Liquid chromatography can be performed in which of the following ways?
1. Gas-liquid chromatography
2. Size exclusion chromatography
3. High-performance liquid chromatography (HPLC)
4. Affinity chromatography
Answer: 3. High-performance liquid chromatography (HPLC)
13. Chromatography cannot be used to purify volatile substances.
a. True
b. False
Answer: b. False
14. Gas chromatography can be performed in which of the following ways?
1. Liquid-solid chromatography
2. Gas-liquid chromatography
3. Affinity chromatography
4. Ion exchange chromatography
Answer: 2. Gas-liquid chromatography
15. Ion exchange chromatography is based on the
1. Size of molecules
2. Polarity of molecules
3. Charge of molecules
4. Density of molecules
Answer: 3. Charge of molecules
16. In which chromatography is the stationary phase more polar than the mobile phase?
1. Normal phase liquid chromatography
2. Reverse phase chromatography
3. Gas chromatography
4. Affinity chromatography
Answer: 1. Normal phase liquid chromatography
17. In column chromatography, the stationary phase is made of ___________, and the mobile phase is made of___________
1. Silica gel; organic solvent
2. Alumina; water
3. Paper; water
4. Glass beads; air
Answer: 1. Silica gel; organic solvent
18. In column chromatography, the number of distribution planes is directly proportional to ________ and inversely proportional to ______.
1. Column length; particle size
2. Particle size; column length
3. Solubility; density
4. Density; solubility
Answer: 2. Particle size; column length
MCQ on Chromatography
There are 30+ MCQ on Chromatography questions compiled in the PDF given below. These MCQ on Chromatograph are based on wide-ranging topics of chromatography. Aspirants can get an idea of what is chromatography, its types, and its applications.
Our MCQ on Chromatography Questions & Answers PDF prepares you for various exams, tests, and interviews. Moreover, each MCQ on Chromatography Question has a correct answer along with a detailed explanation.
Solve the below MCQ on chromatography to prepare better for any entrance exam. It is very beneficial to solve MCQs before exams for multiple reasons. You can practice, analyze, and understand concepts while solving the MCQ on Chromatography. If you wish to learn more and Get an idea about MCQ on chromatography by solving the ones compiled below for your practice.
1. Chromatography is a physical method that is used to separate__________
(a) Simple mixtures
(b) Complex mixtures
(c) Viscous mixtures
(d) Metals
Answer: B
2. Which force is involved in the Chromatography?
(a) Hydrogen bonding
(b) London force
(c) Electric static force
(d) All of the above
Answer: D
3. Ion exchange chromatography is based on the_____________
(a) Electrostatic attraction
(b) Electrical mobility of ionic species
(c) Adsorption chromatography
(d) Partition chromatography
Answer: A
4. Chromatography with a solid stationary phase is called_________
(a) circle chromatography
(b) Square chromatography
(c) solid chromatography
(d) adsorption chromatography
Answer: D
5. A combination of paper chromatography and electrophoresis involves______________
(a) Partition chromatography
(b) Electrical mobility of the ionic species
(c) Both (a) and (b)
(d) None of these
Answer: C
6. The pattern on the paper in chromatography is called
(a) chroming
(b) Chroma
(c) Chromatograph
(d) Chromatogram
Answer: D
7. In reverse phase chromatography, the stationary phase is made______________
(a) Non-polar
(b) Polar
(c) Both a and b
(d) None of these
Answer: A
8. The components that have a small value of K have an affinity for______________
(a) mobile phase
(b) stationary phase
(c) no phase
(d) solution
Answer: B
9. Which technique is also known as color writing?
(a) NMR
(b) Mass spectroscopy
(c) Chromatography
(d) All of the above
Answer: C
10. Which of the following HPLC detectors is used as a bulk property or general-purpose detector?
(a) Electrochemical detector
(b) Fluorescence detector
(c) UV-Visible detector
(d) Evaporative Light scattering detector
Answer : D
11. Thin layer chromatography is__________
(a) Partition chromatography
(b) Electrical mobility of ionic species
(c) Adsorption chromatography
(d) None of the above
Answer: C
12. In which chromatography the stationary phase is more polar than the mobile phase?
(a) Ion exchange chromatography
(b) Normal phase chromatography
(c) Reversed chromatography
(d) Size exclusion chromatography
Answer: B
13. Which of the following is used as a spraying reagent in paper chromatography?
(a) conc. HCl
(b) NaCl solution
(c) Ninhydrin solution
(d) CuSO4 solution
Answer: C
14. In gas chromatography, the basis for the separation of the components of the volatile material is the difference in________
(a) Partition coefficients
(b) Conductivity
(c) Molecular weight
(d) Molarity
Answer: A
15. In which type of chromatography, the stationary phase is held in a narrow tube, and the mobile phase is forced through it under pressure?
(a) Column chromatography
(b) Planar chromatography
(c) Liquid chromatography
(d) Gas chromatography
Answer: A
16. In chromatography, the stationary phase can be ___________ supported on a solid.
(a) Solid or liquid
(b) Liquid or gas
(c) Solid only
(d) Liquid only
Answer: A
17. What is Eluent?
(a) is a liquid solution
(b) is a liquid solution that is a result of Elution.
(c) It is a solvent that is used for the separation of absorbed material from the stationary phase.
(d) None of the above
Answer: C
18. In chromatography, which of the following can the mobile phase be made of?
(a) Solid or liquid
(b) Liquid or gas
(c) Gas only
(d) Liquid only
Answer: B
19. Chromatogram is__________
(a) Solute concentration vs Elution time
(b) Solute concentration vs Elution volume
(c) A and B
(d) None of the above
Answer: C
20. In thin-layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
(a) Solid, liquid
(b) Liquid, liquid
(c) Liquid, gas
(d) Solid, gas
Answer: A
21. What is the Analyte?
(a) Substance for separation
(b) Substance for impurity
(c) A and B
(d) None of the above
Answer: A
22. Which of the following is used as a carrier gas in gas chromatography__________
(a) Carbon dioxide
(b) Oxygen
(c) Helium
(d) Methane
Answer: C
23. In size exclusion chromatography, solute molecules are separated based on _________
(a) Molecular geometry and size
(b) Molecular composition
(c) Molecular phase
(d) Molecular formula
Answer: A
24. Ion exchange chromatography is based on?
(a) Electrostatic attraction
(b) Electrical mobility of ionic species
(c) Partition chromatography
(d) Adsorption chromatography
Answer: B
25. In Thin layer chromatography, the stationary phase is made of _________, and the mobile phase is made of _________
(a) Solid, liquid
(b) Liquid, liquid
(c) Liquid, gas
(d) Solid, gas
Answer: A
26. The basis of the technique of chromatography for separating components of a mixture is?
(a) the differing movement of particles of different masses in an electrical field
(b) the interaction of the components with stationary and mobile phases
(c) the absorption of infrared radiation by the components.
(d) the deflection of charged particles in a magnetic field.
Answer: B
27. HPLC is an abbreviation for?
(a) High-Profit Liquid Chromatography
(b) High-Pressure Liquid Chromatography
(c) Higher Performance Low Chromatography
(d) Higher Profit Low Chromatography
Answer: B
28. Which of the following techniques would be most useful to identify as well as quantify the presence of a known impurity in a drug substance?
(a) NMR
(b) MS
(c) IR
(d) HPLC
Answer: D
29. Liquid chromatography can be performed in which of the following ways?
(a) Only in columns
(b) Only on plane surfaces
(c) Either in columns or on plane surfaces
(d) Neither in columns nor on plane surfaces
Answer: C
30. The process of passing a mobile phase through a chromatography column is called which one of the following?
(a) Flushing
(b) Washing
(c) Elution
(d) Partitioning
Answer: C
31. Which of the following techniques is used to study the three-dimensional structure of a molecule?
(a) Infra-red spectroscopy
(b) Mass spectrometry
(c) UV-visible spectroscopy
(d) X-ray crystallography
Answer: D
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MCQ on Chromatography Important Terms
- Analyte: It is a product of a chromatographic process.
- Analytical Chromatography: It is used to determine the presence and concentration of analyte in the sample.
- Preparative Chromatography: It is used for the purification of substances for specific purposes (for analysis).
- Chromatogram: It is a visual representation or results of the chromatographic process. Each substance corresponds to a certain peak on the chromatogram.
- Chromatograph: Instrument for carrying out chromatography.
- Holding time: The time during which the analyte passes through the chromatographic system under certain conditions.
- Stationary phase: Substance that is attached to the column or the panel, on whose surface the substances are separated.
- Mobile phase: Phase that is moving in a certain direction. It can be a liquid or gas. The mobile phase moves through a column that carries the sample to be separated.