MCQ on Electrophoresis with Answers

MCQ on Electrophoresis with Answers: Electrophoresis is the separation of charged macromolecules in space when they move under the influence of an electric field. The key characteristic that determines the possibility of separation is the mobility of the macromolecule. In general, mobility depends on the physical size of the molecule, its charge, and the characteristics of the external stationary environment in which the molecule moves.

MCQ on Electrophoresis

To assist students in achieving high scores in the Electrophoresis Unit, we have presented an MCQ on electrophoresis aligned with the new exam pattern. Alongside each set, comprehensive answers are provided for clarity. These Electrophoresis MCQ with Answers serve as a valuable resource, offering students insights into the framing and presentation of multiple-choice questions in the exam.

The accompanying PDF format of MCQ on Electrophoresis with Answers is designed to facilitate improved time management, enabling students to efficiently tackle Electrophoresis MCQ during the examination.

1. What is electrophoresis Mcq?
a. A type of protein structure
b. A laboratory technique for separating DNA, RNA, or proteins based on their size and charge
c. A type of chemical reaction
d. A form of nuclear energy
Answer: b. A laboratory technique for separating DNA, RNA, or proteins based on their size and charge

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2. Which technique separates charged particles using electric field?
a. Hydrolysis
b. Electrophoresis
c. Protein synthesis
d. Protein denaturing
Answer: b. Electrophoresis

3. Why do people use electrophoresis?
a. To bake cakes
b. To separate biomolecules based on their properties
c. To generate electricity
d. To study astronomy
Answer: b. To separate biomolecules based on their properties

4. Who found electrophoresis?
a. Thomas Edison
b. James Clerk Maxwell
c. Arne Tiselius
d. Marie Curie
Answer: c. Arne Tiselius

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5. Who is the father of gel electrophoresis?
a. Watson and Crick
b. Rosalind Franklin
c. Fred Sanger
d. Richard Altmann
Answer: c. Fred Sanger

6. Electrophoresis apparatus consists of:
a. Bunsen burners and test tubes
b. Pipettes and Petri dishes
c. Gel tank, power supply, and electrodes
d. Microscopes and slides
Answer: c. Gel tank, power supply, and electrodes

7. Agarose gel electrophoresis voltage:
a. Depends on the phase of the moon
b. Is inversely proportional to the buffer concentration
c. Should be as high as possible for faster results
d. Is applied based on the size of the molecules being separated
Answer: d. Is applied based on the size of the molecules being separated

8. Electrophoresis cannot be used to separate:
a. DNA fragments
b. RNA molecules
c. Proteins
d. Elements in the Periodic Table
Answer: d. Elements in the Periodic Table

9. Factors that affect gel electrophoresis:
a. Atmospheric pressure and humidity
b. The color of the gel
c. Temperature and pH
d. The scientist’s mood
Answer: c. Temperature and pH

10. Agarose is a polysaccharide extracted from:
a) Algae
b) Bacteria
c) Fungi
d) Plants
Answer: a) Algae

11. How is nucleic acid separated using agarose gel electrophoresis?
a. By boiling the sample
b. By freezing the sample
c. By size and charge
d. By adding more nucleotides
Answer: c. By size and charge

12. How are molecules separated in agarose gel electrophoresis based on?
a. Alphabetical order
b. Atomic weight
c. Size and charge
d. Solubility in water
Answer: c. Size and charge

13. The migration of nucleic acid molecules during gel electrophoresis is driven by:
a. Gravity
b. Centrifugal force
c. Electrical charge
d.Temperature gradient
Answer: c. Electrical charge

14. Who invented agarose gel?
a. Rosalind Franklin
b. Michael Faraday
c. Anselm Payen
d. Richard Altmann
Answer: c. Anselm Payen

15. What is the full form of TAE buffer?
a. Total Amine Extract
b. Tris-Acetate-EDTA
c. Transcriptional Amplification Element
d. Thymine-Adenine Exchange
Answer: b. Tris-Acetate-EDTA

16. Why ethidium bromide is used in gel electrophoresis?
a. To add color to the gel
b. To increase the viscosity of the gel
c. To stain DNA and make it visible under UV light
d. To enhance the conductivity of the gel
Answer: c. To stain DNA and make it visible under UV light

17. What is the full form of EtBr?
a. Ethylene Bromide
b. Ethidium Bromide
c. Ester Brevibacterium
d. Electron Binding Resin
Answer: b. Ethidium Bromide

18. Why TAE buffer is used in electrophoresis?
a. To enhance the taste of the sample
b. To maintain a stable pH environment
c. To increase the temperature of the gel
d. To accelerate the migration of DNA
Answer: b. To maintain a stable pH environment

19. What is the use of an electrophoretic unit?
a. To make coffee
b. To separate charged particles based on their mobility
c. To launch satellites into space
d. To measure atmospheric pressure
Answer: b. To separate charged particles based on their mobility

20. What is the principle of electrophoresis?
a. Separation based on color
b. Separation based on taste
c. Separation based on size and charge
d. Separation based on temperature
Answer: c. Separation based on size and charge

21. Which disease is diagnosed by electrophoresis test?
a. Influenza
b. Diabetes
c. Sickle cell anemia
d. Tuberculosis
Answer: c. Sickle cell anemia

Above MCQ on Electrophoresis is provided here based on the curriculum and as per the latest syllabus, these MCQs are prepared. Practicing these MCQ on Electrophoresis objective questions will guide students to have a quick revision for all the topics present in each chapter and prepare themselves for the final semester exams.

Electrophoresis MCQ with Answers:

MCQ on Electrophoresis with answers are provided here for biotechnology and microbiology students. These MCQs are based on the biotechnology curriculum and correspond to the most recent syllabus. By practicing these MCQ on Electrophoresis, students will be able to quickly review all of the ideas covered in the chapter and prepare for the semester examinations as well as other competitive exams.

1. The speed of migration of ions in an electric field depends upon:
A. Shape and size of molecule
B. The magnitude of charge and shape of a molecule
C. The magnitude of charge shape and mass of a molecule
D. The magnitude of charge and mass of a molecule
Answer: b. The magnitude of charge and shape of a molecule

2. For the separation of DNA by electrophoresis, which of the following methods is commonly used?
A. Agarose – vertical
B. Agarose – horizontal
C. PAGE – vertical
D. PAGE – horizontal
Answer: b. Agarose – horizontal

3. Which of the following is the purpose of gel electrophoresis?
A. Construction of recombinant DNA by joining with cloning vectors
B. Separation of DNA fragments according to their size
C. Cutting of DNA into fragments
D. Isolation of DNA molecule
Answer: b. Separation of DNA fragments according to their size

4. Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________
A. An anionic detergent
B. A cationic detergent
C. A non-ionic detergent
D. An anion exchanger
Answer: a. An anionic detergent

5. Function of β-mercaptoethanol in SDS-PAGE is__________.
A. To give negative charges to amino acids in the proteins
B. For the oxidation of disulfide bonds in the proteins
C. For the reduction of disulfide bonds in the proteins
D. For breaking hydrogen bonds in the proteins
Answer: c. For the reduction of disulfide bonds in the proteins

6. Immunoelectrophoresis can be used to study_________
A. Correspondence between antigens and antibodies
B. Determine the purity of an antigen or antibody
C. Determine if a protein is present in the complex
D. All of these
Answer: D. All of these

7. Agarose gel is suitable for__________
A. Separation of immune complexes, nucleic acids and nucleoproteins
B. Identification of immune complexes, nucleic acids and nucleoproteins
C. Purification of immune complexes, nucleic acids and nucleoproteins
D. All of these
Answer: D. All of these

8. The advantages of polyacrylamide gel are_________
A. Good mechanical strength and elasticity
B. Transparent, high chemical stability
C. Both A and B
D. None of these
Answer: C. Both A and B

9. External factors affecting electrophoresis are_________
A. Electric field strength
B. pH of solution
C. Ionic strength of solution
D. All of these
Answer: D. All of these

10. Polyacrylamide gel is a synthetic gel, its advantages are________
A. Good mechanical strength, low elasticity, no electroosmosis effect, and high resolution
B. Good mechanical strength, high elasticity, electroosmosis, and high resolution
C. Good mechanical strength, high elasticity, electroosmosis, low resolution
D. Good mechanical strength, high elasticity, no electroosmosis, and high resolution
Answer: D. Good mechanical strength, high elasticity, no electroosmosis, and high resolution

11. The characteristics of two-dimensional electrophoresis technology are
A. Highest resolution
B. Most Informative
C. Stable pH
D. All of these
Answer: D. All of these

12. Among the following descriptions of capillary gel electrophoresis, which one is correct
A. Electrophoresis is performed by moving the gel on the plate into a capillary tube as a support.
B. Gels are porous and act like molecular sieves
C. Ability to separate based on differences in mass-to-charge ratio and molecular volume of components to be measured
D. All of These
Answer: D. All of These

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