Preservation of Industrial Important Microorganisms
Preservation of Industrial microorganisms MCQ Questions and Answers:
1. Which scientists gave the details of the techniques of preservation?
(1) Jacob and Monod
(2) William and Wilkins
(3) Boliver and Rodriguez
(4) Kirsop and Doyle
Answer: 4
2. What is the temperature of liquid nitrogen (°C)?
(1) -140°C
(2) -130°C
(3) -170°C
(4) -196°C
Answer: 4
3. The preservation by liquid Nitrogen is called as___________
(1) Cryopreservation
(2) Lyophilization
(3) Freeze-drying
(4) Desiccation
Answer: 1
4. Which of the following method used for the preservation of spores_______
(1) Drying
(2) Freezing
(3) Liquid Nitrogen
(4) Agar Slopes
Answer: 1
5. Which of the following is NOT the technique of preservation?
(1) storage on agar slants
(2) storage under liquid nitrogen
(3) dried cultures
(4) storage in water
Answer: 4
6. What is the best method for preserving a bacterial culture for 10 years or longer?
(1) Making glycerol stock (10-20% glycerol)
(2) Drying
(3) Freezing
(4) None of these
Answer: 1
7. Which of the following is not a Cryoprotective agent?
(1) Sodium hypochlorite
(2) DMSO
(3) Glycerol
(4) Sucrose
Answer: 1
8. Which of the following method is used for the preservation of microbial cultures?
(1) Refrigeration,
(2) Paraffin method
(3) Cryopreservation
(4) Lyophilization
(5) All of these
Answer: 5
9. Which of the following method involves sublimation of cell water?
(1) Cryopreservation
(2) Lyophilization
(3) Dried Culture
(4) Desiccation
Answer: 1
10. What is the best method for storing bacterial cultures for short periods?
(1) Cryopreservation
(2) Freezing
(3) Dried Culture
(4) Desiccation
Answer: 2
11. The preservation of agar slopes has an expiration of 6 months and the agar needs to be changed every 6 months. Which of the following can be used to extend sub-culturing to 1 year?
(1) Paraffin oil
(2) DMSO
(3) Glycerol
(4) Loamy Soil
Answer: 1
12. Which of the following is/are used in dried culture preservation?
(1) Sand
(2) Soil
(3) Sand and Soil
(4) Slits
Answer: 3
13. Which of the following is NOT the advantage of lyophilization?
(1) Dissolution of product
(2) Processed in aseptic conditions
(3) Handling and processing time increases
(4) Do not use heat
Answer: 3
14. Which of the following method of preservation is more suitable and used widely?
(1) Dried Cultures
(2) Lyophilization
(3) Salt Storage
(4) Storage in liquid Nitrogen
Answer: 2
15. What scientific instrument is used to measure the turbidity of a microbial population?
(1) Turbidimetry
(2) Photometers
(3) Spectrophotometers
(4) All of these
Answer: 4
16. Which of the following is are examples of Cryoprotective agents?
(1) Ethylene glycol
(2) dimethyl sulfoxide (DMSO)
(3) Glycerol
(4) All of these
Answer: 4
17. Which of the following is a function of cryoprotective agents?
(1) for long-term preservation of cultures
(2) prevents cell damage due to ice crystal formation
(3) prevents the formation of ice
(4) to trap the liquid nitrogen
Answer: 2
18. Glycerol and DMSO have been used for decades by cryobiologists to reduce ice formation in________
(1) sperm
(2) oocytes
(3) embryos
(4) all of the above
Answer: 4
19. Which method is mainly applied for the preservation of Sporulating microorganisms?
(1) Storage in sterile soil
(2) Paraffin method
(3) Cryopreservation
(4) Lyophilization
Answer: A
