MCQ on SDS PAGE with Answers
MCQ on SDS PAGE are available online for easy access. These MCQ on SDS PAGE cover all the concepts explained in the Electrophoresis Unit, so that students can test themselves as to their level of preparation for semester exams. Below is the list of MCQ on SDS PAGE. Let’s check it out.
MCQ on SDS PAGE
These questions help students to understand the concepts in a better way. They also help to test students’ knowledge. Practicing these SDS PAGE MCQ questions is one of the ideal methods to prepare and crack semester examinations. Cross-check answers with the detailed explanations given below on MCQ on SDS PAGE.
1. What is the role of SDS in SDS-PAGE?
a. Stabilize protein structure
b. Separate proteins based on size
c. Enhance protein binding
d. None of the above
Answer: b. Separate proteins based on size
Explanation: SDS (Sodium Dodecyl Sulfate) in SDS-PAGE denatures proteins and imparts a negative charge, facilitating separation based on size during electrophoresis.
Read: MCQ on Gel Electrophoresis
2. Which of the following buffer is used in SDS-PAGE?
a. Tris-Acetate
b. Tris-Glycine
c. Tris-HCl
d. Tris-EDTA
Answer: b. Tris-Glycine
Explanation: Tris-Glycine buffer is commonly used in SDS-PAGE for maintaining pH stability during electrophoresis.
Read: MCQ on Electrophoresis
3. Why is TEMED used in SDS-PAGE?
a. Enhance protein staining
b. Initiate polymerization of acrylamide
c. Neutralize SDS
d. Accelerate gel drying
Answer: b. Initiate polymerization of acrylamide
Explanation: TEMED (N,N,N’,N’-Tetramethylethylenediamine) is a catalyst that helps in the polymerization of acrylamide and bisacrylamide to form the gel matrix.
4. Which of the following gel is used in SDS-PAGE?
a. Agarose gel
b. Polyacrylamide gel
c. Gelatin gel
d. Collagen gel
Answer: b. Polyacrylamide gel
Explanation: SDS-PAGE utilizes polyacrylamide gels for separating proteins based on their molecular weights.
5. What is the full form of TEMED?
a. Tris-Ethylene Matrix Ethyl Diamine
b. Tetramethylethylenediamine
c. Triethylamine-Methanol-Ethylene Diamine
d. Tetraethylmethylenediamine
Answer: b. Tetramethylethylenediamine
Explanation: TEMED stands for N,N,N’,N’-Tetramethylethylenediamine.
6. Which technique is also called SDS-PAGE?
a. Southern blotting
b. Western blotting
c. Northern blotting
d. Protein electrophoresis
Answer: d. Protein electrophoresis
Explanation: SDS-PAGE is a technique used for protein electrophoresis.
7. What is the principle of SDS?
a. Protein denaturation
b. DNA fragmentation
c. RNA synthesis
d. Enzyme activation
Answer: a. Protein denaturation
Explanation: SDS denatures proteins by disrupting their structure, unfolding them into linear chains.
8. What is the principle of PAGE gel?
a. Protein separation based on size
b. DNA separation based on charge
c. RNA separation based on shape
d. Enzyme concentration measurement
Answer: a. Protein separation based on size
Explanation: Polyacrylamide Gel Electrophoresis (PAGE) separates biomolecules based on size, with SDS-PAGE specifically used for proteins.
9. Can SDS-PAGE be used for DNA?
a. Yes
b. No
c. Only with additional chemicals
d. Depends on the size of the DNA
Answer: b. No
Explanation: SDS-PAGE is primarily designed for proteins and may not effectively separate DNA.
10. What is the purpose of SDS-PAGE?
a. Quantify enzyme activity
b. Separate and analyze proteins
c. Measure DNA concentration
d. Identify RNA structure
Answer: b. Separate and analyze proteins
Explanation: SDS-PAGE is specifically used to separate and analyze proteins based on their molecular weights.
11. What are the advantages of SDS-PAGE?
a. High resolution and rapid results
b. Efficient separation of nucleic acids
c. Minimal sample denaturation
d. Only suitable for small protein sizes
Answer: a. High resolution and rapid results
Explanation: SDS-PAGE provides a high-resolution separation of proteins and delivers results relatively quickly.
12. Why is DNA not treated with SDS?
a. DNA is not negatively charged
b. SDS inhibits DNA replication
c. DNA does not denature like proteins
d. DNA is already in a linear form
Answer: c. DNA does not denature like proteins
Explanation: Unlike proteins, DNA does not undergo denaturation in the presence of SDS, making SDS-PAGE less suitable for DNA separation
13. Why is polyacrylamide gel used in SDS-PAGE?
a. To enhance protein binding
b. To visualize DNA structures
c. To separate proteins based on size
d. To accelerate gel drying
Answer: c. To separate proteins based on size
Explanation: Polyacrylamide gel is used in SDS-PAGE to achieve size-based separation of proteins during electrophoresis.
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